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c rel  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology c rel
    C Rel, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+rel/c-Rel+Antibody/pm41904146-250-110-112
    Average 95 stars, based on 803 article reviews
    c rel - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: EZH2 promotes endometriosis progression through estrogen receptor and TNFα expression
    Article Snippet: Antibodies for ERα (sc-8005), c-Rel (sc-6955), Ki-67 (sc-101861), NF1 (sc-74444) and TNFα (sc-52746) were sourced from Santa Cruz Biotechnology.

    Incubation:

    Article Title: O-GlcNAcylation regulates microglial neuroinflammation in Parkinson's disease.
    Article Snippet: Western Blotting AR TI CL E IN P RE SS Equal amounts of protein (30 μg) were separated on 10% or 15% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham). .. Membranes were blocked with 5% nonfat milk in PBST for 1 h at room temperature, then incubated overnight at 4°C with primary antibodies (1:1,000) against various targets, including O-GlcNAc (sc-59623, RL2, Santa Cruz, Dallas, TX, USA), OGT (sc-74546, Santa Cruz), OGA (14771-1-AP, Proteintech, Rosemont, IL, USA), GFAT2 (A15374, Abclonal, Woburn, MA, USA) tyrosine hydroxylase (T2928,TH, Sigma-Aldrich), β-actin (sc28365, Santa Cruz), p-p65 (ab86229, Abcam, Cambridge, UK), p65 (sc-8008, Santa Cruz), p-IκB (sc-8404, Santa Cruz), IκB (sc-371, Santa Cruz), iNOS (610328, BD Bioscience, San Jose, CA, USA), COX2 (A3560, Abclonal), p-CREB (ab32096, Abcam), CREB (sc-377154, Santa Cruz), TLR4 (A0007, Abclonal), Arg1 (A4923, Abclonal), CD163 (A26411PM, Abclonal), CD86 (A16805, Abclonal), CD63 (A6554, Abclonal), c-Rel (sc-6955, Santa Cruz), p-STAT3 (9145, Cell Signaling Technology, Danvers, MA, USA), STAT3 (sc-8019, Santa Cruz), p-ERK (9101, Cell Signaling Technology), ERK (A16686, Abclonal), PPARγ (sc-7273, Santa Cruz), NLRP3 (sc134306, Santa Cruz), ASC (sc-514414, Cell Signaling Technology), cleaved caspase 1 (89332, Cell Signaling Technology), and caspase 1 (24232, Cell Signaling Technology). .. Membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies (Invitrogen) for 1 h at RT.

    Article Title: Alloxan attenuates glucosamine-induced NF-κB activation and proinflammatory gene expression under normoglycemic conditions
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies against O -GlcNAc (CTD110.6; Covance, Berkeley, CA, USA), inducible nitric oxide synthase (iNOS; BD Transduction Laboratories, Lexington, KY, USA), cyclooxygenase-2 (COX-2; Cayman Chemical, Ann Arbor, MI), NF-κB subunits p65, c-Rel, p50, p52, RelB (Santa Cruz Biotechnology, Dallas, TX, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Danvers, MA, USA) as a loading control. .. After three washes with TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Amersham Biosciences) diluted 1:10,000 in TBST for 1 hour at room temperature.

    Bioprocessing:

    Article Title: Disruption of Epithelial Barrier Integrity via Altered GILZ/c-Rel/RACK1 Signaling in Inflammatory Bowel Disease.
    Article Snippet: Background and Aims: Given the role of Receptor for Activated C Kinase 1 (RACK1) in both immune cell activation and in the maintenance of the intestinal epithelial barrier integrity, we investigated whether it was involved in inflammatory bowel disease (IBD).. Methods: RACK1 expression was analyzed in intestinal mucosal samples of healthy and IBD patients, in mice with chemically induced colitis, and in diseased in vitro 2D and 3D coculture models by luciferase assay, reverse transcription-quantitative PCR, Western blotting, immunofluorescence, and immunohistochemistry.. Based on our finding that glucocorticoid-induced leucine zipper (GILZ or tsc22d3) positively correlates with RACK1 expression in IBD patients, GILZ knockout mice and cell silencing experiments were performed.

    Article Title: Disruption of Epithelial Barrier Integrity via Altered GILZ/c-Rel/RACK1 Signaling in Inflammatory Bowel Disease
    Article Snippet: Dextran sulfate sodium (DSS) salt (colitis grade, 36 000–50 000 M wt) (PubChem CID: 53788809) was purchased from MP Biomedicals (Solon, OH, USA). .. The anti-human RACK1 (sc-17754), c-Rel (sc-6955), SRC-1 (sc-32789), ZO-1 (sc-33725), and GAPDH (sc-47724) mouse monoclonal antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). .. The mouse monoclonal anti-β-tubulin antibody (T0198) was from Sigma Aldrich (St Louis, MO, USA).

    Control:

    Article Title: Alloxan attenuates glucosamine-induced NF-κB activation and proinflammatory gene expression under normoglycemic conditions
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies against O -GlcNAc (CTD110.6; Covance, Berkeley, CA, USA), inducible nitric oxide synthase (iNOS; BD Transduction Laboratories, Lexington, KY, USA), cyclooxygenase-2 (COX-2; Cayman Chemical, Ann Arbor, MI), NF-κB subunits p65, c-Rel, p50, p52, RelB (Santa Cruz Biotechnology, Dallas, TX, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Danvers, MA, USA) as a loading control. .. After three washes with TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Amersham Biosciences) diluted 1:10,000 in TBST for 1 hour at room temperature.



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